The measurement results of the dual beam UV visible spectrophotometer are more stable and accurate

UV visible spectrophotometer is a widely used analytical instrument in laboratories, used to measure the absorption characteristics of substances in the UV and visible light bands. Among them, the dual beam UV visible spectrophotometer has become an important tool in scientific research and industrial testing due to its high accuracy, stability, and wide range of applications. This article will provide a detailed introduction to its working principle, technical characteristics, advantages, and applications in multiple fields.

1、 The working principle of a dual beam UV visible spectrophotometer:

1. Light source system: Typically, deuterium lamps (in the ultraviolet region, 190-350 nm) and tungsten lamps (in the visible region, 350-1100 nm) are used as light sources, covering a wide range of wavelengths.

2. Spectral system: Grating or prism decomposes composite light into monochromatic light, and selects specific wavelengths of light through a slit to enter the sample chamber.

3. Beam splitting: The incident light is split into two beams by a beam splitter, one passing through the sample cell and the other passing through the reference cell.

4. Detection and signal processing: Two beams of light are respectively detected by photomultiplier tubes (PMT) or photodiodes, and the system calculates the absorbance of the sample by comparing the intensity difference between the two beams of light.

Due to the dual beam design, real-time correction of light source fluctuations and detector drift can be achieved, resulting in more stable and accurate measurement results.

2、 Technical characteristics of dual beam UV visible spectrophotometer

(1) High precision and stability

-Reduce the impact of light source fluctuations, temperature changes, and detector drift through real-time reference correction.

-Suitable for long-term continuous monitoring, such as enzyme kinetics experiments or chemical reaction process analysis.

(2) Wide wavelength range

-Usually covering 190-1100 nm, it is suitable for the detection of most organic compounds, inorganic ions, and biomolecules.

(3) Quick scanning capability

-By using high-precision grating drive and high-speed data acquisition system, full wavelength scanning can be completed in a few seconds.

(4) Automation and Intelligence

-Modern instruments are equipped with touch screens, computer control, and data analysis software, supporting automatic wavelength switching, multi wavelength measurement, and data processing.

3、 Main application areas

(1) Biochemistry and Molecular Biology

-Nucleic acid and protein quantification: Calculate sample concentration by measuring absorbance at 260 nm (DNA/RNA) and 280 nm (protein).

-Enzyme kinetics research: Monitor changes in absorbance during enzymatic reactions and calculate reaction rates.

(2) Drug analysis

-Drug content determination: such as quantitative analysis of antibiotics and vitamins.

-Dissolution test: Monitor the release rate of drugs in simulated body fluids.

(3) Environmental monitoring

-Water quality analysis: detecting pollutants such as heavy metals (such as lead and mercury), nitrates, COD (chemical oxygen demand), etc. in water.

-Air pollutant monitoring: UV absorption detection of nitrogen dioxide (NO ₂) and ozone (O3).

(4) Materials Science

-Characterization of nanomaterials: Measure the UV visible absorption spectra of quantum dots and metal nanoparticles, and analyze their optical properties.

-Thin film thickness analysis: Calculate the thickness and refractive index of the thin film through interferometric spectroscopy.

(5) Food industry

-Food additive testing: quantitative analysis of preservatives and pigments.

-Nutritional analysis: such as determination of vitamin and carbohydrate content.

The dual beam UV visible spectrophotometer has become a modern laboratory analysis tool due to its high accuracy, stability, and wide range of applications. Whether it is basic research or industrial testing, it can provide reliable data support. In the future, with continuous technological innovation, this instrument will play an important role in more fields, promoting scientific research and industrial progress.